Quantification and Identification of Culturable Airborne Bacteria from Duck Houses
Identifieur interne : 000094 ( Main/Exploration ); précédent : 000093; suivant : 000095Quantification and Identification of Culturable Airborne Bacteria from Duck Houses
Auteurs : Elena Martin [Allemagne] ; Peter Kmpfer [Allemagne] ; Udo Jckel [Allemagne]Source :
- Annals of Occupational Hygiene [ 0003-4878 ] ; 2009-12-30.
Abstract
Employees at agricultural working places are often exposed to complex bioaerosols. Investigations of bioaerosols in duck houses revealed concentrations of cultivable bacteria between 0.4 and 3105 colony forming units (CFU) m3 on tryptone soy agar, 0.3 and 2105 CFU m3 on actinomycetes isolation agar, and 0.8 and 5103 CFU m3 on Middlebrook agar, respectively, when incubated at 25C. At an incubation temperature of 37C, 0.63102 CFU m3 were counted on MacConkey agar and 0.32103 CFU m3 on Middlebrook agar, and the concentrations of bacteria on glycerolarginine agar and oatmeal agar incubated at 50C varied between 0.1 and 2103 and 1 and 7103 CFU m3, respectively. In addition, high concentrations of cells were observed by fluorescence microscope quantification of cell counts after 4,6-diamidino-2-phenylindol staining with 38107 cells m3. A total of 213 colonies with different morphological appearance were selected and the isolated pure cultures were identified at the genus level using the 16S rRNA gene sequence analyses. In summary, 19 different genera of Actinobacteria, four genera of the Firmicutes, one genus of the Bacteroidetes, and five genera of the Proteobacteria were identified. Several isolates represent new phylogenetic lineages. Based on 16S rRNA gene analyses, some isolates were most closely related to Cellulosimicrobium funkei, Corynebacterium falsenii, Corynebacterium xerosis, Mycobacterium arupense, and Staphylococcus epidermidis, which have been grouped into Risk group 2 of biological agents and may cause negative pulmonary health effects. These bacterial species were present in high concentrations up to 104 CFU m3. For this reason, we recommend an adequate personal breathing protection at these working places.
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DOI: 10.1093/annhyg/mep088
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Investigations of bioaerosols in duck houses revealed concentrations of cultivable bacteria between 0.4 and 3105 colony forming units (CFU) m3 on tryptone soy agar, 0.3 and 2105 CFU m3 on actinomycetes isolation agar, and 0.8 and 5103 CFU m3 on Middlebrook agar, respectively, when incubated at 25C. At an incubation temperature of 37C, 0.63102 CFU m3 were counted on MacConkey agar and 0.32103 CFU m3 on Middlebrook agar, and the concentrations of bacteria on glycerolarginine agar and oatmeal agar incubated at 50C varied between 0.1 and 2103 and 1 and 7103 CFU m3, respectively. In addition, high concentrations of cells were observed by fluorescence microscope quantification of cell counts after 4,6-diamidino-2-phenylindol staining with 38107 cells m3. A total of 213 colonies with different morphological appearance were selected and the isolated pure cultures were identified at the genus level using the 16S rRNA gene sequence analyses. In summary, 19 different genera of Actinobacteria, four genera of the Firmicutes, one genus of the Bacteroidetes, and five genera of the Proteobacteria were identified. Several isolates represent new phylogenetic lineages. Based on 16S rRNA gene analyses, some isolates were most closely related to Cellulosimicrobium funkei, Corynebacterium falsenii, Corynebacterium xerosis, Mycobacterium arupense, and Staphylococcus epidermidis, which have been grouped into Risk group 2 of biological agents and may cause negative pulmonary health effects. These bacterial species were present in high concentrations up to 104 CFU m3. For this reason, we recommend an adequate personal breathing protection at these working places.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Berlin</li></region><settlement><li>Berlin</li></settlement></list><tree><country name="Allemagne"><region name="Berlin"><name sortKey="Martin, Elena" sort="Martin, Elena" uniqKey="Martin E" first="Elena" last="Martin">Elena Martin</name></region><name sortKey="Jckel, Udo" sort="Jckel, Udo" uniqKey="Jckel U" first="Udo" last="Jckel">Udo Jckel</name><name sortKey="Jckel, Udo" sort="Jckel, Udo" uniqKey="Jckel U" first="Udo" last="Jckel">Udo Jckel</name><name sortKey="Kmpfer, Peter" sort="Kmpfer, Peter" uniqKey="Kmpfer P" first="Peter" last="Kmpfer">Peter Kmpfer</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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